M. Petrovic et al., STRUCTURAL AND FUNCTIONAL HOMOLOGY BETWEEN THE 29 KD RAT-LIVER NUCLEOPROTEIN AND THE HIGH-MOBILITY GROUP-1 PROTEIN, Molecular biology reports, 23(2), 1996, pp. 79-85
A 29 kD soluble rat liver nucleoprotein (p29) has increased binding af
finity for the hormone responsive element (RE) of the rat haptoglobin
(Hp) gene during the acute-phase reaction. In this work the possibilit
y of its structural and functional homology to the high mobility group
1 (HMG1) nonhistone protein constituent of chromatin was examined. Th
e results of two-dimensional gel electrophoresis, Southwestern and Wes
tern immunoblot analyses, showed that p29 and HMG1 are homologous prot
ein species. On the basis of in vitro and in vivo phosphorylation/deph
osphorylation experiments, we discuss the modulatory role of phosphate
groups in view of the structure and function of p29.