The cornea, dermis and tendon have extracellular matrix architectures
with differences in fibril diameter, packing and organization. An earl
y step in fibril assembly is the formation of a striated fibril of dis
crete length (segment). Fibril segments were isolated from developing
chicken cornea, dermis and tendon by physical disruption and the struc
ture characterized. In all three tissues, intact but relatively short
fibril lengths were isolated. These segments were asymmetric, having l
ong (alpha) and short (beta) tapered ends. They were also centrosymmet
ric with respect to molecular packing. Segments isolated from 12- to 1
6-day corneas, dermis and tendons had identical structures, but their
lengths and diameters were distinct. We propose that the increase in l
ength is, at least in part, the result of lateral associations of adja
cent segments. In the developing tendon, there is a rapid increase in
length and diameter between day 16 and 17, while in the dermis the inc
rease is more linear with respect to time. In the cornea, the fibril s
egments grow longer, but their diameters remain constant. Disruption o
f corneas in phosphate-buffered saline yielded larger diameter segment
s than seen in situ, while tendon or dermis maintained tissue-specific
diameters. When corneas were disrupted in buffers that stabilized the
water layer associated with the collagen molecules or containing the
corneal proteoglycans, then tissue-specific diameters were maintained.
These data suggest differences in the stabilization of segments durin
g growth in tissues where diameter increases versus those where diamet
er remains constant, and this may be related to collagen-proteoglycan
interactions.