MULTICOLOR SPECTRAL KARYOTYPING OF HUMAN-CHROMOSOMES

The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic impo rtance. Whole-genome scanning by spectral karyotyping allowed instanta neous visualization of defined emission spectra for each human chromos ome after fluorescence in situ hybridization, By means of computer sep aration (classification) of spectra, spectrally overlapping chromosome -specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.