CYTOKINES INDUCED BY SENDAI-VIRUS IN HUMAN PERIPHERAL-BLOOD LEUKOCYTES

Human peripheral blood leukocytes (hPBL) are a rich source of natural leukocyte interferon (IFN-alpha) when treated with Sendai virus, Senda i virus treatment of hPBL will also result in significant production o f several chemokines and cytokines such as macrophage inflammatory pro tein-la (MIP-1 alpha), MIP-1 beta, RANTES, tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and IL-8, in a time-dependent way, A significant amount of MCP-1 is constitutively produced in overnight culture of leukocytes, The most abundant cytokine is IFN-alpha, which is induced to its maximum level approximately 11-15 h after addition of Sendai virus, The amount of IFN-alpha induced at 15 h after Sendai virus treatment is more than 16-fold higher than those of MIP-1 alpha, MIP-1 beta, and RANTES, IFN-alpha is also induced more than 60-fold h igher than TNF-alpha and IL-8, The amount of IL-6 induced is approxima tely 400-fold less than IFN-alpha, Limited amounts of other cytokines such as IL-1 alpha, IL-1 beta, macrophage colony-stimulating factor, T NF-beta, and IFN-gamma are also induced in Sendai virus-treated hPBL, No measurable amount of granulocyte-macrophage colony-stimulating fact or, granulocyte colony-stimulating factor, leukemia inhibitory factor, IL-2, IL-3, IL-4, IL-5, IL-7, IL-10, IL-11, or IL-12 was induced in t he supernatant of Sendai virus-treated hPBL.