GLYCOPROTEIN-H (GLL GP108) AND GLYCOPROTEIN-L FORM A FUNCTIONAL COMPLEX WHICH PLAYS A ROLE IN PENETRATION, BUT NOT IN ATTACHMENT, OF BOVINEHERPESVIRUS-1/

The glycoproteins of bovine herpesvirus 1 (BHV-1) play important roles in the interactions between virions and target cells. A 108 kDa glyco protein, designated gII or gp108, has been identified by two different panels of monoclonal antibodies, The gII- and gp108-specific monoclon al antibodies were shown to react with the same protein, which was ide ntified by N-terminal sequencing as the homologue of herpes simplex vi rus type 1 (HSV-1) gH. When BHV-1 gH was purified by immunoadsorbent c hromatography, gL was co-purified. The gH-gL complex induced the produ ction of antibodies that neutralized virus infectivity and inhibited v irus penetration, Affinity-purified gH-gL did prevent penetration, but not attachment of BHV-1, which suggests that the gH-gL complex is ess ential for penetration of BHV-1 into susceptible cells.