INTERCELLULAR CALCIUM WAVES IN NEURONS

Citation
Ac. Charles et al., INTERCELLULAR CALCIUM WAVES IN NEURONS, Molecular and cellular neurosciences, 7(5), 1996, pp. 337-353
Citations number
56
Categorie Soggetti
Neurosciences
ISSN journal
10447431
Volume
7
Issue
5
Year of publication
1996
Pages
337 - 353
Database
ISI
SICI code
1044-7431(1996)7:5<337:ICWIN>2.0.ZU;2-4
Abstract
Spontaneous intercellular Ca2+ waves were observed in groups of neuron s in two different culture preparations: primary mouse cortical neuron s and GT1-1 immortalized neurons. Waves of increased intracellular Ca2 + concentration propagated at rates of 100-200 mu m/s over as many as 200 cells and were abolished by the removal of extracellular calcium, by nimodipine, by tetrodotoxin, and by the gap junction inhibitor octa nol. A sister clone of the GT1 line, GT1-7 neurons, showed no intercel lular Ca2+ waves and were found to have a significantly lower level of connexin26 mRNA than the GT1-1 line. Although we cannot definitively rule out a role for synaptic communication, we propose that intercellu lar Ca2+ waves in cultured neurons are generated by Ca2+ influx caused primarily by the propagation of depolarization via gap junctions. Int ercellular Ca2+ signaling via gap junctions may represent an important mechanism for nonsynaptic neuronal signaling.