Spontaneous intercellular Ca2+ waves were observed in groups of neuron
s in two different culture preparations: primary mouse cortical neuron
s and GT1-1 immortalized neurons. Waves of increased intracellular Ca2
+ concentration propagated at rates of 100-200 mu m/s over as many as
200 cells and were abolished by the removal of extracellular calcium,
by nimodipine, by tetrodotoxin, and by the gap junction inhibitor octa
nol. A sister clone of the GT1 line, GT1-7 neurons, showed no intercel
lular Ca2+ waves and were found to have a significantly lower level of
connexin26 mRNA than the GT1-1 line. Although we cannot definitively
rule out a role for synaptic communication, we propose that intercellu
lar Ca2+ waves in cultured neurons are generated by Ca2+ influx caused
primarily by the propagation of depolarization via gap junctions. Int
ercellular Ca2+ signaling via gap junctions may represent an important
mechanism for nonsynaptic neuronal signaling.