EXPRESSION IN ESCHERICHIA-COLI AND PURIFICATION OF THE ACTIVE AUTOPROTEASE P20 OF CLASSICAL SWINE FEVER VIRUS

Citation
G. Muyldermans et al., EXPRESSION IN ESCHERICHIA-COLI AND PURIFICATION OF THE ACTIVE AUTOPROTEASE P20 OF CLASSICAL SWINE FEVER VIRUS, Virus genes, 13(2), 1996, pp. 135-142
Citations number
21
Categorie Soggetti
Genetics & Heredity",Virology
Journal title
ISSN journal
09208569
Volume
13
Issue
2
Year of publication
1996
Pages
135 - 142
Database
ISI
SICI code
0920-8569(1996)13:2<135:EIEAPO>2.0.ZU;2-Q
Abstract
The genes for Flaviviridae structural proteins are located at the 5' t erminus of the genome, while the 3' terminus contains the genes for th e non-structural proteins. The first protein product of the large ORF of pestiviruses, the p20 protein! is however a non-structural protein which possess an autoproteolytic activity. Here we report the cloning of the p20/p14 genes behind the strong Trc promotor and expression of the p20 at high levels in E. coli. The autoprotease p20 was responsibl e for its own release from the nascent polyprotein in E. coil and was further purified by chromatography techniques.