TAURINE-CONJUGATED AND GLYCINE-CONJUGATED BILE-ACIDS IN THE PRERUMINANT GOAT - IDENTIFICATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A reversed-phase high performance liquid chromatography technique has been developed for the identification of taurine- and glycine-conjugat ed bile acids in the bile of preruminant goats. The mobile phase consi sted of two solvents: acetonitrile (A) and 5 mM potassium phosphate bu ffer (pH 4.5) (B). Samples (10 mu 1) were eluted with a linear gradien t in which acetonitrile was increased from 25 to 35%, and from 35 to 4 5%, at 10 min intervals. Flow rate was 1.0 ml min(-1), and bile acids were detected at 200 nm. This simple high resolution technique was hig hly reproducible, involved a minimum of straightforward sample treatme nt, and required a short chromatographic development time. The techniq ue will be of use in the systematic identifications of bile acids in p reruminants.