ISOLATION OF GLYCOMACROPEPTIDE FROM SODIUM CASEINATE HYDROLYSATE SOLUTION BY ULTRAFILTRATION

Studies were carried out to selectively hydrolyze sodium caseinate to produce glycomacropeptide (GMP). After acid precipitation of the non G MP components, the resultant crude GMP solution was concentrated with different molecular weight cut-off (MWCO) ultrafiltration (UF) membran es. It was shown that 30,000 MWCO UF membrane process produced crude G MP with the highest purity (50.09%). The presence of ionic interaction s on ultrafiltration transmission is thought to affect the purity of t he product. Further purification of the crude GMP by cation-exchange c hromatography showed that the amino acid composition of GMP isolated c losely resembled its theoretical value. It is possible to produce GMP from sodium caseinate by UF on a large scale basis. If highly purified product is desired, cation-exchange chromatography may be used as an additional purification step.