H. Adams et al., A METHOD FOR THE ARTIFICIAL INOCULATION OF SUGAR-BEET WITH RAMULARIA-BETICOLA UNDER FIELD CONDITIONS, Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 103(3), 1996, pp. 321-323
Artificial inoculation of sugar beet with Ramularia beticola under fie
ld conditions can be conducted by the use of in vitro prepared inoculu
m. The fungus is grown on PDA at 18 degrees C and diffuse daylight. A
homogenate of the non sporulating cultures is sprayed at sugar beet gr
owth stage 39 on the beet leaves. First symptoms occurred 14 dpi in 19
94 and 16 dpi in 1995, respectively. Disease increased due to secondar
y infections. The described method might allow the screening of sugar
beet for resistance to R. beticola after a controlled inoculation.