APPLICATION OF THE DAVIDON-FLETCHER-POWELL ALGORITHM TO THE RESOLUTION OF MULTICOMPONENT MIXTURES USING UV-VIS SPECTROPHOTOMETRY

The Davidon-Fletcher-Powell (DFP) iterative algorithm was used for the simultaneous quantitation of the components of complex mixtures from their UV-vis spectra. First, the effect of noise, overlap between stan dard spectra and the starting point for resolving numerically generate d spectra were investigated. Then, spectra for the pure solutions of t he mixture components were used to apply the method to the resolution of ternary and quaternary mixtures of active principles and pharmaceut ical preparations. Samples were also quantified by using a set of stan dard spectra which included not only those of the pure components, but also some for other substances. The proposed method effectively quant ifies the actual mixture components.