HYDROGEN-PEROXIDE ASSAY BY USING ENHANCED CHEMILUMINESCENCE OF THE LUMINOL-H2O2-HORSERADISH PEROXIDASE SYSTEM - COMPARATIVE-STUDIES

p-Iodophenol, p-coumaric acid, phenol and aniline are enhancers of the luminol-H2O2-horseradish peroxidase chemiluminescence. These enhanced and unenhanced chemiluminescences were used to assay low concentratio ns of hydrogen peroxide. The curves of chemiluminescence intensity max ima against hydrogen peroxide concentration were fitted to third order polynomial regressions, which permit a very good approach to the expe rimental results. Double the sensitivity (RSD less than or equal to 4. 3%) and a slightly lower detection limit (e.g. 0.08 mu M with p-coumar ic acid of 0.18 mu M without enhancer) were obtained with enhanced che miluminescence than with unenhanced chemiluminescence.