NATURAL ANTHRACENONE SUBCELLULAR-DISTRIBUTION AND EFFECTS ON NADPH-CYTOCHROME P450 REDUCTASE MICROSOMAL ACTIVITY

Natural anthracenone subcellular distribution and effects on NADPH-cyt ochrome P450 reductase microsomal activity. Subcellular distribution s tudy of a natural anthracenone (T-514) isolated from Karwinskia humbol dtiana showed to be homogeneous on subcellular (nuclear, mitochondrial , peroxisomal and microsomal) fractions prepared from rat liver treate d with an acute dose of T-514. These results indicate that T-514 can p ass easily through subcellular compartment membranes and an absence of selectivity for some subcellular organelles. A significant increase o f protein on liver homogenates and NADPH-cytochrome P450 reductase mic rosomal activity indicates that T-514 may act as a microsomal enzymati c inducer. In addition, this enzymatic specific activity increment cou ld be due to the interaction of T-514 with the microsomal redox cyclin g.