BIODISTRIBUTION OF COCAINE DURING PERINATAL, PERIOD IN RATS

Differences in cocaine-induced central nervous system toxicity in preg nant and postpartum rats have been observed. For example, a 20 mg/kg, intraperitoneal dosage of cocaine that was tolerated during pregnancy caused tonic-clonic convulsions and death during the postpartum period . In the present st;dy, biodistribution of cocaine during different pe rinatal periods was investigated to understand the differences in toxi city. Nonpregnant, 22-day pregnant, and postpartum rats were injected with H-3 cocaine (12mg/20 mu ci/kg, i.v.). Rats were sacrificed under ether anesthesia at predetermined time intervals. Brain, serum, fetal brain and placenta (from pregnant rats) samples were isolated, solubil ized, and counted in a scintillation counter for radioactivity. Peak l evels of cocaine were observed at 15 and 60 minutes after injection in maternal serum and brain, respectively, in 22-day pregnant rats. Howe ver, peak levels of cocaine were observed at 30 minutes after the inje ction in placental and fetal brain tissues from 22-day pregnant rats. Cocaine level of brain was elevated in all postpartum rats compared to nonpregnant and pregnant rats. These results indicated that higher le vel of cocaine is distributed to brain after delivery compared to the nonpregnant and pregnant rats which may explain increased CNS toxicity during postnatal period.