ANALYSIS OF STARVATION CONDITIONS THAT ALLOW FOR PROLONGED CULTURABILITY OF VIBRIO-VULNIFICUS AT LOW-TEMPERATURE

The response of the estuarine human pathogen Vibrio vulnificus to star vation for carbon, nitrogen or phosphorus, or all three nutrients simu ltaneously (multiple-nutrient), was examined with respect to the maint enance of culturability during incubation at low temperature. V. vulni ficus showed similar survival patterns during starvation for the indiv idual nutrients when kept at 24 degrees C. On the other hand, cultures prestarved at 24 degrees C and then shifted to 5 degrees C maintained culturability at low temperature in a starvation-condition-dependent manner. Carbon and multiple-nutrient starvation were indistinguishable in their ability to mediate maintenance of culturability in the cold, Prolonged starvation for phosphorus had a similar effect, but nitroge n starvation did not allow for maintenance of culturability. Extracell ular factors produced during starvation were not observed to have an e ffect on the culturability of cells incubated at low temperature. Prot ein synthesis during starvation for individual nutrients was analysed by two-dimensional PAGE of pulse-labelled proteins, Carbon and multipl e-nutrient starvation gave nearly identical protein induction patterns involving at least 34 proteins, indicating that carbon starvation det ermines both responses. Nitrogen starvation for 1 h induced 24 protein s, while phosphorus starvation induced a set of 10 proteins after 1 h and about 40 proteins after 18 h. It is suggested that starvation for carbon or phosphorus induces maintenance of culturability of V. vulnif icus incubated at low temperature via the synthesis of distinct sets o f starvation-specific proteins.