The development of any industrial application of a microbial process s
hould include the study of medium composition. Experimental design and
surface response technologies have been applied to Flavobacterium deh
ydrogenans growth, concluding that, in the range of conditions used, c
oncentration of glucose, phosphate buffer and yeast extract are the mo
st significant factors in biomass production. Response surface methodo
logy provides a model describing the relationship between these factor
s and produced biomass.