THE IN-VIVO GLYCO-OXIDATION OF ALPHA-GLOBIN AND BETA-GLOBIN INVESTIGATED BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY

Matrix-assisted laser desorption/ionization (MALDI) has been employed for the determination of molecular weights of alpha- and beta-globins obtained from blood samples of healthy and diabetic subjects, Glycated species, originating from the reaction of glucose with the proteins, are easily evident, The MALDI measurements allowed one to distinguish between simple glycation and glyco-oxidation processes of haemoglobin, In fact, together with the products arising from simple glucose conde nsation on the two proteins, compounds at different oxidation levels w ere evident. Due to its high specificity, MALDI must be considered a v aluable tool for diagnosis in the diabetes field.