CHARACTERIZATION OF A SUNFLOWER SEED ALBUMIN WHICH ASSOCIATES WITH OIL BODIES

Authors
THOYTS PJE NAPIER JA MILLICHIP M STOBART AK GRIFFITHS WT TATHAM AS SHEWRY PR
Citation
Pje. Thoyts et al., CHARACTERIZATION OF A SUNFLOWER SEED ALBUMIN WHICH ASSOCIATES WITH OIL BODIES, PLANT SCI, 118(2), 1996, pp. 119-125
Citations number
23
Categorie Soggetti
Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
01689452 → ACNP
Volume
118
Issue
2
Year of publication
1996
Pages
119 - 125
Database
ISI
SICI code
0168-9452(1996)118:2<119:COASSA>2.0.ZU;2-X
Abstract
An M(r) 22 500 protein was purified from isolated oil bodies from sunf lower cotyledons. N-terminal amino acid sequencing showed that this pr otein belonged to the 2S albumin group of storage proteins. A correspo nding cDNA clone encoded a preproprotein, comprising two mature 2S alb umin proteins one of which corresponded to the oil-body associated alb umin. In contrast, the second albumin encoded by the cDNA was related to components purified from a total albumin fraction by RP-HPLC.