DETERMINATION OF THE SIZE AND DEGREE OF ACETYL SUBSTITUTION OF OLIGOSACCHARIDES FROM NEISSERIA-MENINGITIDIS GROUP-A BY LONSPRAY MASS-SPECTROMETRY

Authors
CESCUTTI P BIGIO M GUARNIERI V
Citation
P. Cescutti et al., DETERMINATION OF THE SIZE AND DEGREE OF ACETYL SUBSTITUTION OF OLIGOSACCHARIDES FROM NEISSERIA-MENINGITIDIS GROUP-A BY LONSPRAY MASS-SPECTROMETRY, Biochemical and biophysical research communications, 224(2), 1996, pp. 444-450
Citations number
15
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
224
Issue
2
Year of publication
1996
Pages
444 - 450
Database
ISI
SICI code
0006-291X(1996)224:2<444:DOTSAD>2.0.ZU;2-D
Abstract
The capsular polysaccharide produced by Neisseria meningitidis group A has the following structure: [GRAPHICS] This polysaccharide was parti ally hydrolysed with acetic acid, and the oligomers obtained were sepa rated by fast performance liquid chromatography. Six fractions were co llected and characterised by ionspray mass spectrometry in the positiv e ion mode. This soft ionisation technique established the size of the obtained oligosaccharides and the degree of O-acetyl substitution for each fraction. (C) 1996 Academic Press, Inc.