PURIFICATION AND PARTIAL CHARACTERIZATION OF A FATTY-ACID-BINDING PROTEIN FROM THE YEAST, YARROWIA-LIPOLYTICA

Authors
DELLANGELICA EC ERMACORA MR SANTOME JA
Citation
Ec. Dellangelica et al., PURIFICATION AND PARTIAL CHARACTERIZATION OF A FATTY-ACID-BINDING PROTEIN FROM THE YEAST, YARROWIA-LIPOLYTICA, Biochemistry and molecular biology international, 39(3), 1996, pp. 439-445
Citations number
15
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
39
Issue
3
Year of publication
1996
Pages
439 - 445
Database
ISI
SICI code
1039-9712(1996)39:3<439:PAPCOA>2.0.ZU;2-N
Abstract
A low-molecular-mass fatty acid-binding protein was isolated from the cytosol of the yeast Yarrowia lipolytica. Purification was achieved by a two-step procedure involving size-exclusion and cation-exchange chr omatography. The isolated protein exists as a monomer of 15 kDa, is ba sic and has a blocked N-terminus. Internal amino acid sequencing sugge sts that this protein may belong to a novel class of fatty acid-bindin g proteins.