CAPILLARY ELECTROPHORETIC ENANTIOSEPARATION OF SELEGILINE, METHAMPHETAMINE AND EPHEDRINE USING A NEUTRAL BETA-CYCLODEXTRIN EPICHLORHYDRIN POLYMER

This paper describes the development of a capillary zone electrophoret ic method for chiral separation of three basic compounds of the selegi line synthetic pathway: ephedrine, methamphethamine and selegiline. Th e method developed allows one to separate the studied compounds in one run using a neutral beta-cyclodextrin epichlorhydrin polymer. The eff ect of various experimental parameters, such as chiral selector concen tration, concentration and composition of background electrolyte, pH, temperature, and the addition of some organic solvents, on the resolut ion and migration time is discussed. For selegiline and methamphetamin e, it is possible, under optimal conditions, to quantify less than 0.5 % of the minor isomer in an excess of the major one.