ANALYSIS OF STEROIDS .50. DERIVATIZATION OF KETOSTEROIDS FOR THEIR SEPARATION AND DETERMINATION BY CAPILLARY ELECTROPHORESIS

Authors
GOROG S GAZDAG M KEMENESBAKOS P
Citation
S. Gorog et al., ANALYSIS OF STEROIDS .50. DERIVATIZATION OF KETOSTEROIDS FOR THEIR SEPARATION AND DETERMINATION BY CAPILLARY ELECTROPHORESIS, Journal of pharmaceutical and biomedical analysis, 14(8-10), 1996, pp. 1115-1124
Citations number
16
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Journal of pharmaceutical and biomedical analysis → ACNP
ISSN journal
07317085
Volume
14
Issue
8-10
Year of publication
1996
Pages
1115 - 1124
Database
ISI
SICI code
0731-7085(1996)14:8-10<1115:AOS.DO>2.0.ZU;2-A
Abstract
4-Ene-3-ketosteroids and 17-ketosteroids were quantitatively transform ed into the corresponding hydrazones using Girard P and T reagents, re spectively. The positively charged derivatives were separated by capil lary electrophoresis. The spectrophotometric characteristics of the de rivatives permitted their sensitive detection in the 230-280 nm range. The steroids investigated included nortestosterone and its phenylprop ionate, norethisterone and its oenanthate, d,l-norgestrel, dehydroepia ndrosterone, androstenedione and ethisterone.