SAMPLE PREPARATION FOR TAXOL AND CEPHALOMANNINE DETERMINATION IN VARIOUS ORGANS OF TAXUS SP

Solid-phase extraction and preparative thin-layer chromatography were applied as sample preparation techniques for the purification of crude extracts from twigs and needles of various Taxus species as well as f or the isolation of taxol and cephalomannine for further reversed phas e high performance liquid chromatography analysis. Significant differe nces in the contents of taxanes examined were found. The preparative c hromatographic methods used were compared and evaluated as routine and reproducible procedures for the rapid isolation and determination of taxol and cephalomannine in plant extracts.