L. Zivanovic et al., COMPARISION OF HIGH-PERFORMANCE AND THIN-LAYER CHROMATOGRAPHIC METHODS FOR THE ASSAY OF LIDOCAINE, Journal of pharmaceutical and biomedical analysis, 14(8-10), 1996, pp. 1229-1232
Reversed-phase high-performance liquid chromatographic (HPLC) and thin
-layer chromatographic (TLC)-UV densitometric methods were developed f
or the quality control of lidocaine hydrochloride bulk drug and its in
jection solutions. The HPLC method used an RP-18 reversed-phase column
with methanol-water-1% phosphoric acid-hexylamine (30:70:100:1.4, v/v
/v/v) as the mobile phase and detection at 254 nm, with a capacity fac
tor k' = 0.8, The TLC-UV densitometric method was performed on silica
gel plates using diisopropyl ether-acetone-diethylamine (85:10:5, v/v/
v) as the developing solvent and UV detection at 254 nm. The response
was linear up to 10 mu g ml(-1) (HPLC) and 8 mg ml(-1) (TLC). The RSD
of the peak areas was 1.71% for HPLC and 0.55% for TLC, with recoverie
s in the range 99.6-100.2% for HPLC and 99.2-100.7% for TLC.