HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC ANALYSIS OF HYDROLYZED TINIDAZOLE SOLUTIONS .1. DEVELOPMENT AND VALIDATION METHOD

A stability-indicating high performance thin layer chromatography meth od for analyzing hydrolyzed tinidazole solutions using silica gel plat es was developed and validated. The mobile phase used was methanol-die thyl ether-chloroform (1:9:3, v/v/v) allowing small changes in its com position. Detection was at 314 nm. R(f) values being 0.1-0.4, baseline resolution was achieved for tinidazole and the hydrolysis products. T he analytes were stable on the sorbent and could be precisely and accu rately measured in the range 20-170 ng per band.