THE ALGORITHM OF ESTIMATION OF THE K-M VALUES FOR PRIMERS IN DNA-SYNTHESIS CATALYZED BY HUMAN DNA-POLYMERASE-ALPHA

Authors
KOLOCHEVA TI MAKSAKOVA GA ZAKHAROVA OD NEVINSKY GA
Citation
Ti. Kolocheva et al., THE ALGORITHM OF ESTIMATION OF THE K-M VALUES FOR PRIMERS IN DNA-SYNTHESIS CATALYZED BY HUMAN DNA-POLYMERASE-ALPHA, FEBS letters, 399(1-2), 1996, pp. 113-116
Citations number
25
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
399
Issue
1-2
Year of publication
1996
Pages
113 - 116
Database
ISI
SICI code
0014-5793(1996)399:1-2<113:TAOEOT>2.0.ZU;2-M
Abstract
DNA synthesis with various deoxyribo homo- and heterooligoprimers in t he presence of complementary templates was investigated. The lengtheni ng of d(pN)(n) primers (n = 1-10) by a unit resulted in an increase of the primer affinity and a maximal rate of polymerization. The coeffic ient of the affinity enhancement of primers due to formation of one hy drogen bond between primer and template was found to be 1.35. The depe ndence of the primer affinity and polymerization rate on template-prim er structure in solution was analyzed and the objective laws of the ch anges of the K-M and V-max values were revealed.