Dh. Brown et G. Brumelis, A BIOMONITORING METHOD USING THE CELLULAR-DISTRIBUTION OF METALS IN MOSS, Science of the total environment, 187(2), 1996, pp. 153-161
Samples of the feather moss Hylocomium splendens, were subjected to a
sequential elution procedure in order to quantify the metal content of
the intercellular, extracellular exchangeable, intracellular and part
iculate fractions. Measurements were made on segments of different age
and with increasing distance from a heavy metal pollution source. The
effect of desiccation and storage time following field sampling was s
hown to modify the cellular location of elements, but rehydration coul
d partly reverse such responses. Ni and Pb were shown to be equally ef
fective for the elution of extracellular exchangeable Zn supplied unde
r laboratory conditions. The value of the sequential elution procedure
for heavy metal biomonitoring studies is discussed.