AMYLIN GIVEN BY CENTRAL OR PERIPHERAL ROUTES DECREASES GASTRIC-EMPTYING AND INTESTINAL TRANSIT IN THE RAT

The effect of rat amylin on gastric emptying and intestinal transit in the rat was examined. Amylin administered intracerebroventricularly ( 1, 2, 2.5 or 4 mu g/rat) produced the maximal decrease in gastric empt ying and intestinal transit at the dose of 2.5 mu g/rat. Higher doses produced a lower effect. Peripheral administration (25, 50 or 100 mu g /kg) produced dose-dependent effects. Pre-treatment with neostigmine b locked the effect of amylin when it was centrally injected, while the effect of amylin given peripherally was partially reduced. Pre-treatme nt with domperidone decreased the inhibitory effect of peripherally in jected amylin, but no effect was observed when the peptide was central ly injected.