VIDEO MICROSCOPY TO QUANTITATE THE INHOMOGENEOUS EQUILIBRIUM STRAIN WITHIN ARTICULAR-CARTILAGE DURING CONFINED COMPRESSION

The objectives of this study were to develop a method to quantitate th e displacement and strain fields within articular cartilage during equ ilibrium confined compression, and to use the method to determine the variation of the equilibrium confined compression modulus with depth f rom the articular surface in bovine cartilage. The method made use of fluorescently labeled chondrocyte nuclei as intrinsic fiducial markers . Articular cartilage was harvested from the patellofemoral groove of adult bovines and trimmed to rectangular blocks 5 mm long, 0.76 mm wid e, and 500 mu m deep with the articular surface intact. Test specimens were stained with the DNA binding dye Hoechst 33258, placed in a cust om confined compression chamber, and viewed with an epifluorescence mi croscope equipped for video image acquisition. Image processing was us ed to localize fluorescing chondrocyte nuclei in uncompressed and comp ressed (similar to 17%) specimens, allowing determination of the intra tissue displacement profile. Strain was determined as the slope of lin ear regression fits of the displacement data in four sequential 125-mu m-thick layers. Equilibrium strains varied 6.1-fold from the articula r surface through 500 mu m of cartilage depth, with the greatest compr essive strain in the superficial 125-mu m layer and the least compress ive strain in the two deepest 125-mu m layers. Thus, the four successi ve 125-mu m layers have moduli that are 0.44 (superficial), 1.07, 2.39 , and 2.67 (deep) times the apparent modulus for a 500-mu m thick cart ilage sample assumed to be homogeneous.