O. Heidenreich et al., A HAMMERHEAD RIBOZYME CLEAVES ITS TARGET RNA DURING RNA PREPARATION, ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 6(2), 1996, pp. 141-144
Citations number
13
Categorie Soggetti
Medicine, Research & Experimental","Biothechnology & Applied Migrobiology
A chemically modified hammerhead ribozyme was designed to cleave the t
ax RNA of human T cell leukemia virus type I. This ribozyme was exogen
ously delivered to tax-transformed fibroblasts by DOTAP-mediated trans
fection. The analysis of RNA from ribozyme-transfected cells by RNase
protection detected ribozyme cleavage products. However, control exper
iments revealed that the ribozyme did not cleave its target RNA intrac
ellularly but during the course of the RNA isolation. This suggests th
at great care should be exercised when interpreting in vivo results of
ribozyme cleavage.