AMINES THAT TRANSPORT PROTONS ACROSS BILAYER-MEMBRANES - SYNTHESIS, LYSOSOMAL NEUTRALIZATION, AND 2-PHASE PK(A) VALUES BY NMR

It is desirable to be able to control the pH of lysosomes. A collectio n of lipophilic, nitrogenous bases, designed to act as membrane-active , catalytic proton transfer agents, were prepared and their effective pK(a)s measured in a vigorously stirred, two-phase system. One phase w as a phosphate buffer whose pH was varied over the range ca, 1-11. The other was an immiscible, deuterated organic solvent in which the comp ounds preferentially resided even when protonated. When chemical shift changes versus the pH of the buffer were plotted, clear pK(a) curves were generated that are relevant to transmembrane proton transfer beha vior. The two-phase pK(a)s increased with increasing counterion lipoph ilicity and with increasing organic solvent polarity, The compounds we re also tested for their ability to neutralize the acidity of lysosome s, a model for other acidic vesicles involved in drug sorting. The mos t successful of these, imidazole 6a, has > 100 times the neutralizing power of ammonia, a standard lysosomotropic amine, causing a 1.7 unit rise in lysosomal pH of RAW cells at 0.1 mM, compared to a 0.2 and 1.4 unit rise for ammonium chloride at 0.1 and 10 mM, respectively.