INHIBITION OF MOTILITY AND INVASION OF HUMAN LUNG-CANCER CELLS BY INVASION INHIBITING FACTOR-2

The motility and invasion of cancer cells are basic requirements for t he establishment of distant metastases. In this study, we examined the effect of invasion inhibiting factor 2 (IIF2), a motility/invasion re gulatory agent, on the motility, invasion, growth and basement membran e attachment of human lung cancer cells. IIF2 significantly reduced ce ll dissociation, colony scattering and invasion induced by the motogen ic factor, HGF/SF. Western and Northern analyses showed these cells to be positive for the HGF/SF receptor c-met. These effects were blocked by an anti-IIF2 antibody. IIF2 did not affect the growth and attachme nt of lung cancer cells to the basement membrane. It is concluded ther efore that invasion inhibiting factor 2 is an inhibitor of human lung cancer cell motility and invasion in vitro and this may bear some impo rtance in the construction of anti-metastatic therapies.