LOW-TEMPERATURE RESISTANCE OF HIGHER-PLANTS IS SIGNIFICANTLY ENHANCEDBY A NONSPECIFIC CYANOBACTERIAL DESATURASE

A broad-specificity Delta 9 desaturase gene was cloned from the cyanob acterium Anacystis nidulans. The enzyme introduces a cis-double bond a t the Delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduce d into tobacco plants under transcriptional control of the cauliflower mosaic virus 35S promoter, and the enzyme was targeted into plastids by the transit peptide of the pea RuBisCO small subunit, The transgeni c plants had a highly reduced level of saturated fatty acid content in most membrane lipids and exhibited a significant increase in chilling resistance.