NOVEL BIOTRANSFORMATIONS ON PERACYLATED POLYPHENOLICS BY LIPASES IMMOBILIZED IN MICROEMULSION-BASED GELS AND ON CARBOHYDRATES BY CANDIDA-ANTARTICA LIPASE

The lipases from porcine pancreas (PPL) and Candida cylindracea (CCL), solubilized in AOT [sodium bis-(2-ethylhexyl) sulphosuccinate] water- in-oil (w/o) microemulsions and supported on gelatin have been used fo r selective deacylations of perpropanoates and peracetates of differen t classes of polyphenolics. In addition, lipase from Candida antartica (Novozym 435) has been used for regioselective propanoylation of 2-de oxy-D-ribose and D-ribose, the products on subsequent acetylation with acetic anhydride/pyridine afforded the corresponding tri- and tetraac yl derivatives, universal synthones for the synthesis of 2'-deoxyribon ucleosides and ribonucleosides, respectively. An attempt has; also bee n made to synthesise acylated erythrooxetanose using the same methodol ogy.