H. Schneider et al., CLONING AND CHARACTERIZATION OF ANOTHER LIGNIN PEROXIDASE GENE FROM THE WHITE-ROT FUNGUS PHANEROCHAETE-CHRYSOSPORIUM, Biological chemistry Hoppe-Seyler, 377(6), 1996, pp. 399-402
Lignin peroxidase (LIP) isozymes of Phanerochaete chrysosporium are en
coded by a large family of closely related genes, whose total number i
s still unknown. Among genomic clones, obtained using the polymerase c
hain reaction to clone the LIP gene LPOA from Phanerochaefe chrysospor
ium strain BKM-F 1767, another LIP gene was found. This gene, HG3, sho
wed more than 95% nucleotide homology to those LIP gene variants which
encode LIP isozyme H8. The gene encodes a protein of 372 amino acids,
including the typical leader sequence for secretion, that is identica
l to the LIP isozyme H8 except for 6 amino acid substitutions.