Mm. Verheggen et al., EXPRESSION OF LIPOCORTINS IN HUMAN BRONCHIAL EPITHELIAL-CELLS - EFFECTS OF IL-1-BETA, TNF-ALPHA, LPS AND DEXAMETHASONE, Mediators of inflammation, 5(3), 1996, pp. 210-217
In this study, we investigated the expression of lipocortin I and II (
annexin I and II) in the human bronchial epithelium, both in vivo and
in vitro. A clear expression of lipocortin I and II protein was found
in the epithelium in sections of bronchial tissue, In cultured human b
ronchial epithelial cells we demonstrated the expression of lipocortin
I and II mRNA and protein using Northern blotting, FACScan analysis a
nd ELISA. No induction of lipocortin I or II mRNA or protein was obser
ved after incubation with dexamethasone, Stimulation of bronchial epit
helial cells with IL-1 beta, TNF-alpha or LPS for 24 h did not affect
the lipocortin I or II mRNA or protein expression, although PGE(2) and
6-keto-PGF(1 alpha) production was significantly increased. This IL-1
beta- and LPS-mediated increase in eicosanoids could be reduced by de
xamethasone, but was not accompanied by an increase in lipocortin I or
II expression, In human bronchial epithelial cells this particular gl
ucocorticoid action is not mediated through lipocortin I or II: induct
ion.