A QUANTITATIVE TEST (HCMV-HYBRID-CAPTURE(TM)) TO DETECT HUMAN CYTOMEGALOVIRUS DNA IN THE BLOOD OF IMMUNOCOMPROMISED PATIENTS COMPARED WITH ANTIGENEMIA AND POLYMERASE CHAIN-REACTION

Authors
LAZZAROTTO T CAMPISI B DALMONTE P GALLI S SPEZZACATENA P GUGLIELMI P LANDINI MP
Citation
T. Lazzarotto et al., A QUANTITATIVE TEST (HCMV-HYBRID-CAPTURE(TM)) TO DETECT HUMAN CYTOMEGALOVIRUS DNA IN THE BLOOD OF IMMUNOCOMPROMISED PATIENTS COMPARED WITH ANTIGENEMIA AND POLYMERASE CHAIN-REACTION, The New microbiologica, 19(3), 1996, pp. 193-201
Citations number
17
Categorie Soggetti
Microbiology
Journal title
The New microbiologica
ISSN journal
11217138 → ACNP
Volume
19
Issue
3
Year of publication
1996
Pages
193 - 201
Database
ISI
SICI code
1121-7138(1996)19:3<193:AQT(TD>2.0.ZU;2-U
Abstract
A quantitative assay is necessary to differentiate between low and hig h Cytomegalovirus load in the blood, a high load frequently being corr elated with clinical disease. A new method for the quantitative determ ination of viral DNA (HCMV-hybrid-capture(TM)) was compared with antig enemia and PCR. Hybrid-capture proved to be a simple and rapid method for the quantitative determination of viral load in the blood. It is l ess sensitive than PCR and antigenemia, but seems to correlate with cl inical symptoms as well as the antigenemia test and better than non qu antitative PCR.