Faecal samples from children between 3 months and 5 yrs were investiga
ted for the presence of methanogenic bacteria. Methanobacteria were no
t detected in faecal samples obtained from children under 27 months of
age. At 27 months only one subject harbored methanobacteria; the numb
er of methanobacteria hosts subsequently increased with age, with an i
ncidence of 40% at 3 years and 60% at 5 years. The appearance of metha
nobacteria was not directly related to introduction of particular food
s in the child's diet, which after 2 years becomes similar to that of
Italian adults. These dietary changes could give rise to some physical
-chemical modifications of the enteric lumen thus causing the conversi
on of the intestinal flora to an adult pattern and, in most subjects,
the development of methanobacteria.