EVALUATION AND CHARACTERIZATION OF A PORCINE SMALL-INTESTINE CDNA LIBRARY - ANALYSIS OF 839 CLONES

A porcine small intestine directionally cloned cDNA library was constr ucted in the vector lambda Zap II. Clones were hybridized with total l abeled cDNA such that putative high-copy number transcripts could be d ifferentiated from middle- and low-copy number transcripts prior to se lection and characterization by DNA sequencing. More than 2000 non-hyb ridizing and 242 hybridizing clones were collected. In total, 839 clon es were sequenced from the 3' end of the cDNA, and after inter-clone c omparison, the unique clones were sequenced from the 5' end of the cDN A. The 5' data were used to query the sequence in databases and result ed in the identification of 630 different gene transcripts, of which 6 04 are new porcine genes. The identity of 361 transcripts could be ide ntified from sequence comparison studies. The validity of this semi-ra ndom selection approach was verified by the identification of a large number of unique transcripts.