DISTINCTION OF SPECIES IN AUREOBASIDIUM AND RELATED GENERA BY PCR-RIBOTYPING

Taxonomic markers for differentiation of the anamorph genera Aureobasi dium, Hormonema and Kabatiella were developed using PCR-ribotyping wit h the primers 5.8S-R and LR7 for amplification and the restriction enz ymes AluI, DdeI, HhaI, MspI and RsaI for digestion. Aureobasidium and Hormonema are optimally differentiated with MspI; DdeI is particularly useful to distinguish Aureobasidium, Kabatiella and Selenophoma. Rela tionships of the anamorph genera Aureobasidium, Hormonema and Kabatiel la with the teleomorph genera Pringsheimia and Dothiora are discussed.