THE EFFECT OF A NUCLEOTIDE-NUCLEOSIDE SOLUTION ON HEPATIC REGENERATION AFTER PARTIAL-HEPATECTOMY IN RATS

After hepatectomy, purine and pyrimidine metabolism is a key process i n the synthesis of DNA and RNA and maintaining cellular energy metabol ism. The purpose of this study is to evaluate changes in blood purine and pyrimidine levels after partial hepatectomy and the effect of puri ne and pyrimidine nucleoside solution injection on hepatic regeneratio n under the hypothesis that the rat after partial hepatectomy requires substrates for salvage nucleotide synthesis and changes blood nucleos ide and nucleobase levels. Blood levels of nucleotides, nucleosides, a nd nucleobase by high-performance liquid chromatography method and liv er ATP level by enzymatic analysis, and the effect of preoperative inj ection of nucleoside solution (OG-VI) on hepatic regeneration ratio an d hepatocytes DNA synthesis, were assessed in rats after 70% partial h epatectomy. Decreased liver adenosine triphosphate and increased plasm a xanthine and hypoxanthine after partial hepatectomy indicated an inc rease in catabolism of purine nucleotides in regenerating liver. Plasm a thymidine and cytidine levels increased, then returned to the preval ue, suggesting that the thymidine and cytidine pool was enlarged. OG-V I increased labeling indices of hepatocytes at postoperative d 1 (POD) and hepatic regeneration ratio at POD 14. Blood purine nucleobase and pyrimidine nucleoside levels change after partial hepatectomy and pre operative supply of nucleoside solution is effective for increasing he patocytes DNA synthesis and hepatic regeneration after partial hepatec tomy. (C) Elsevier Science Inc.