ANALYSIS OF COENZYME-Q SYSTEMS, MONOSACCHARIDE PATTERNS OF PURIFIED CELL-WALLS, AND RAPD-PCR PATTERNS IN THE GENUS KLUYVEROMYCES

Analysis of the coenzyme Q system and the monosaccharide pattern of pu rified cell walls were used for species characterization in the genus Kluyveromyces. All the type strains of the genus possess coenzyme Q-6 and the mannose - glucose ('Saccharomyces type') cell wall sugar patte rn. With the help of Random Amplified Polymorphic DNA - Polymerase Cha in Reaction analysis 17 species were separated: K. aestuarii, K. afric anus, K. bacillisporus, K. blattae, K. delphensis, K. dobzhanski, K. l actis (anamorph Candida sphaerica), K. lodderae, K. marxianus (syn. K. fragilis, K. bulgaricus, K. cicerisporus, anamorphs Candida macedonie nsis, C. pseudotropicalis, C. kefyr), K. phaffii, K. piceae, K. polysp orus, K. sinensis, K. thermotolerans (syn. K. veronae, anamorph Candid a dattila), K. waltii, K. wickerhamii, K. yarrowii (anamorph Candida t annotolerans). A strain of K. drosophilarum showed with the type strai n of K. lactis only 63% similarity. The strain originally described as the type strain of K. cellobiovorus nom. nud. was excluded from the g enus (Q-9), and found to be conspecific with the type strain of Candid a intermedia.