The possibility of employing antagonistic bacteria for postharvest con
trol of gray mold on pear was studied. Approximately 175 strains of ba
cteria were isolated from beneath the skin of various fruits and veget
ables and 14 of these possessed high levels of antagonistic properties
. They significantly reduced gray mold in cv Conference pear artificia
lly inoculated with Botrytis cinerea and stored at 20 degrees C for at
least 9 days. Two strains, 3PPE (Bacillus pumilus) and 2TOE (Bacillus
amyloliquefaciens), were particularly active. They did not produce an
tibiotic substances and their application was compatible with iprodion
e-based antifungal treatments. The bacterial antagonist mixed with the
fungicide at 50 ppm a.i. resulted in a greater control of the pathoge
n compared to the single ingredient. Holding bacteria-treated fruits a
t 20 degrees C for 24 h before cold storage improved the efficacy of t
he bacteria against gray mold. (C) 1996 Academic Press, Inc.