IDENTIFICATION AND CHARACTERIZATION OF MI CROORGANISM STRAINS BY THE GENOMIC FINGERPRINTING TECHNIQUE WITH BIOTINYLATED PHAGE M13 DNA

Authors
ORESHKOVA SF MANOKHINA OV PUCHKOVA LI REPIN VE ILICHEV AA
Citation
Sf. Oreshkova et al., IDENTIFICATION AND CHARACTERIZATION OF MI CROORGANISM STRAINS BY THE GENOMIC FINGERPRINTING TECHNIQUE WITH BIOTINYLATED PHAGE M13 DNA, Genetika, 32(6), 1996, pp. 740-743
Citations number
17
Categorie Soggetti
Genetics & Heredity
Journal title
Genetika → ACNP
ISSN journal
00166758
Volume
32
Issue
6
Year of publication
1996
Pages
740 - 743
Database
ISI
SICI code
0016-6758(1996)32:6<740:IACOMC>2.0.ZU;2-D
Abstract
To analyze DNA polymorphisms of various bacterial strains, a nonradioa ctive variant of the genomic fingerprinting method was developed. The method was based on the application of biotin-labeled single-stranded phage M13 DNA as a probe. Characteristic patterns of fingerprints obta ined by MvaI, HaeIII, and HinfI restriction enzymes are presented for several species of bacilli and other bacteria. The advantages of this method in microbiology for the identification and characterization of different microbial strains are shown.