EVALUATION OF ANTI-HUMAN ANTIBODIES FOR IMMUNOHISTOCHEMISTRY ON ARCHIVAL NONHUMAN PRIMATE TISSUES

A panel of commercially available antibodies which recognize specific antigens on human tissues was developed for use in immunohistochemistr y on tissues from eight species of nonhuman primates, Antibodies were selected for potential usefulness in diagnostic pathology, and for eff ectiveness in formalin-fixed, paraffin-embedded tissues. Tissues from four species of macaques and four New World monkeys were evaluated. Us ing these antibodies we were able to identify 17/21 antigens examined in all eight species, and 21/21 antigens in the four species of macaqu es. Detailed immunohistochemistry protocols are presented, along with a systematic approach to developing a protocol for a new antibody.