HUMAN ALBUMIN SOLDERS FOR CLINICAL-APPLICATION DURING LASER-TISSUE WELDING

Background and Objective: Fifty percent human albumin solder significa ntly improves weld strength when compared to lower concentrations [Wri ght et al., ASLMS meeting, April, 1995]. We developed a method for pre paring 50% human albumin that may be considered compatible for clinica l applications. Study Design/Materials and Methods: Fifty percent huma n albumin solder was prepared from 25% commercially available human al bumin using a lyophilization technique. Assessment of sterility, visco sity, pH, and peak absorption wavelength were performed. Results: This report describes the methology used to prepare a 50% human albumin so lder that is compatible with clinical use, Maintenance of the structur al integrity of the albumin was confirmed by polyacrylamide gel electr ophoresis. Conclusion: This solder preparation can be used alone or wi th the addition of exogenous chromophores. The final product is steril e, incorporates viral free protocols, maintains high viscosity, and ca n be applied easily during open or laparoscopic procedures. (C) 1996 W iley-Liss, Inc.