DEVELOPMENT AND EVALUATION OF AN ELISA METHOD FOR THE DETERMINATION OF LIPOPROTEIN-LIPASE MASS CONCENTRATION - COMPARISON WITH A COMMERCIAL, ONE-STEP ENZYME-IMMUNOASSAY

developed a non-competitive, enzyme-linked, immunosorbent assay (ELISA ) for the quantitation of lipoprotein lipase (LPL) in human posthepari n plasma using affinity-purified antihuman milk lipoprotein lipase ant ibodies produced in chicken eggs and a monoclonal antibody directed ag ainst human lipoprotein lipase. We compared our ELISA method with a co mmercially available sandwich-enzyme immunoassay (Markit-F LPL EIA Kit , Dainippon Pharmaceutical Co, Ltd. Osaka, Japan). The reference value s for lipoprotein lipase catalytic activity concentration and mass con centration in healthy Finns were determined. Lipoprotein lipase activi ty concentration (mean +/- SD) was 297 +/- 112 U/l in women, and mass concentration as measured by the ELISA method was 1058 +/- 367 mu g/l. The corresponding values for men were 247 +/- 97 U/l and 815 +/- 207 mu g/l, respectively. Across the whole concentration range of the ELIS A method, the control samples' intra- and inter-assay coefficients of variation (CV) were 5.1% and 6.5%, respectively. The correlation betwe en the ELISA and EIA methods was good, r = +0.81. The importance of th e correct standardisation of immunoassays is discussed.