PROTECTIVE EFFECT OF INTERLEUKIN-1 RECEPTOR ANTAGONIST ON OLEIC ACID-INDUCED LUNG INJURY

Objective. To observe the changes of interleukin-l (IL-l), nitric oxid e (NO) and nitric oxide synthase (NOS) in mice with oleic acid-induced acute lung injury (ALI) and the protective effects of interleukin-l r eceptor antagonist Material and Methods. Male Kunming mice were divide d into control, oleic acid and IL-1ra groups. The control group mice w ere injected saline; the oleic acid group mice were injected oleic aci d (0.2ml / kg); and the IL-1ra group mice were injected the IL-1ra (20 mg / kg). Lung index, lung wet-to-dry weight ratio, and total protein, cell analysis, nitric oxide measurement, NOS activity in BALF, lung p athology examination were made after an hour of administration of drug . Results, Preadministration of IL-1ra to the mouse with ALI decreased the lung index, lung wet-to-dry weight ratio and leakage of protein f rom pulmonary capillary, elevated PaO2, and attenuated lung histologic injury. It was found that in bronchoalveolar lavage fluid (BALF), NO amount and lung NOS activity increased in oleic acid group, BALF NO am ount and lung NOS activity decreased obviously after given IL-1ra. Con clusions. This study demonstrated the protective effect of IL-1ra on o leic acid-induced lung injury, NO may participate in the pathological process of lung injury.