UV-B INDUCES DNA-DAMAGE AND DNA-SYNTHESIS DELAY IN THE MARINE DIATOM CYCLOTELLA SP

The effect of UV-B on the occurrence of DNA damage and consequences fo r the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dos e (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused d etectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements an d quantification of the fraction of recently divided cells revealed th at the DNA synthesis rate as well as the rate of cell division were re duced during and shortly after UV-B exposure. Apparently, UV-B irradia tion extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are removed.