ANALYSIS OF THE HEMOLYMPH-PROTEINS FROM THE ACORN-BARNACLE MEGABALANUS-ROSA BY 2-DIMENSIONAL GEL-ELECTROPHORESIS

Authors
MURAMOTO K KURIKI T NOKIHARA K YAKO H KAMIYA H
Citation
K. Muramoto et al., ANALYSIS OF THE HEMOLYMPH-PROTEINS FROM THE ACORN-BARNACLE MEGABALANUS-ROSA BY 2-DIMENSIONAL GEL-ELECTROPHORESIS, Fisheries science, 62(3), 1996, pp. 435-438
Citations number
11
Categorie Soggetti
Fisheries
Journal title
Fisheries science → ACNP
ISSN journal
09199268
Volume
62
Issue
3
Year of publication
1996
Pages
435 - 438
Database
ISI
SICI code
0919-9268(1996)62:3<435:AOTHFT>2.0.ZU;2-2
Abstract
The hemolymph proteins of the acorn barnacle Megabalanus rosa were sep arated by two-dimensional gel electrophoresis. More than 50 proteins w ere observed in the region of acidic (pl: 4-7) with masses of 18-120 k Da. The proteins separated on the gel were electroblotted onto a polyv inylidene difluoride membrane and were sequenced. Several major protei ns were further characterized by amino acid analysis and peptide mappi ng, and identified to be multiple lectins.